Human CellExp™ KITLG /SCF, mouse recombinant

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SKU:
7483-10
$183
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Description

Human CellExp™ KITLG /SCF, mouse recombinant is available at Gentaur for Next week delivery.

Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis and stem cell maintenance.

Biomolecule/Target: KITLG /SCF

Alternates names: KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, SHEP7, KL.

Synonyms: KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, SHEP7, KL.

Background Information: Kit ligand (KITLG) also known as stem cell factor (SCF), mast cell growth factor (MGF), steel factor (SF), which belongs to the SCF family, and is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. KITLG is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF / MGF plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG / SCF binding can activate several signaling pathways. KITLG / SF Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG / SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG / SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG / SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5 - triphosphate. KITLG / SCF acts synergistically with other cytokines, probably interleukins.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

NCBI Gene Symbol: KITLG

Gene ID: 17311

NCBI Accession: P20826

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Additional Information

Size:
10 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Mouse
Recombinant:
Yes
Source:
HEK293 cells
Purity by SDS-PAGE:
≥98%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
<1 EU/μg by LAL method
Activity (Specifications/test method):
N/A
Biological activity:
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5 - 8 ng/ml.
Results:
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5 - 8 ng/ml.
Molecular Weight:
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 19.1 kDa. The predicted N-terminus is Lys 26. DTT-reduced Protein migrates as 19-30 kDa due to different glycosylation.
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized
Handling:
Centrifuge the vial prior to opening.
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