BioVision Products
Human CellExp™ IL-4 Rα, Human Recombinant
- SKU:
- 7101-50
Description
Human CellExp™ IL-4 Rα, Human Recombinant is available at Gentaur for Next week delivery.
A receptor for both interleukin 4 and interleukin 13
Biomolecule/Target: IL-4 Rα
Alternates names: soluble IL-4 receptor alpha, CD124
Synonyms: soluble IL-4 receptor alpha, CD124
Background Information: IL-4 can signal through type I and type II receptor complexes, which share a common gamma chain (γc). The type I receptor contains in addition to the γc an IL-4Rα subunit, whereas the type II receptor contains the IL-13Rα. The secreted extracellular domain of IL-4Rα, called sIL-4Rα, binds IL-4 and antagonizes its activity. It plays an important role in regulating the differentiation of naïve CD4+ T cells and class switching to IgG1 and IgE. Recombinant human sIL-4Rα is a 209 amino acid protein which corresponds to the entire extracellular domain of IL- 4Rα.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to - 80°C
NCBI Gene Symbol: IL4RA
Gene ID: 3566
NCBI Accession: P24394
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥98% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
< 0.1 ng/μg of protein (<1EU/μg). |
Activity (Specifications/test method): |
Determined by its ability to inhibit the IL-4-dependent proliferation of human TF-1 cells. The expected ED50 is ≤ 5.0 ng/ml (in the presence of 0.5 ng/ml of hIL-4), corresponding to a specific activity of ≥ 2 x 105 units/mg. |
Biological activity: |
Determined by its ability to inhibit the IL-4-dependent proliferation of human TF-1 cells. The expected ED50 is ≤ 5.0 ng/ml (in the presence of 0.5 ng/ml of hIL-4), corresponding to a specific activity of ≥ 2 x 105 units/mg. |
Results: |
N/A |
Molecular Weight: |
~24 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized powder |
Handling: |
Centrifuge the vial prior to opening. |