BioVision Products
Human CellExp™ IL-17E, human recombinant
- SKU:
- 7224-10
Description
Human CellExp™ IL-17E, human recombinant is available at Gentaur for Next week delivery.
Induces NF-κB activation and stimulates the production of IL8
Biomolecule/Target: IL-17E
Alternates names: IL-17E, IL-25, Interleukin-25
Synonyms: IL-17E, IL-25, Interleukin-25
Background Information: Interleukin 25 (IL25) also known as Interleukin-17E (IL-17E), is a distinct member of the IL17 cytokine family comprised of at least six members sharing a conserved cysteine-knot structure but divergent at the N-terminus. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. IL25 can induce NF-κB activation, and stimulate the production of IL8. Both this cytokine and IL17B are ligands for the cytokine receptor IL17RB. IL-25 induces the production of other cytokines, including IL-4, IL-5 and IL-13 in multiple tissues, which stimulate the expansion of eosinophils. This cytokine is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract. IL-25 can kill some types of breast cancer cells.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: IL-17E
Gene ID: 64806
NCBI Accession: Q9H293
Additional Information
Size: |
10 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK 293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
< 0.1 ng/μg of protein (<1EU/μg). |
Activity (Specifications/test method): |
The activity is determined by the dose-dependent production of IL-8 by human PBMCs. The expected ED50 for this effect is 12-25 ng/ml. |
Biological activity: |
The activity is determined by the dose-dependent production of IL-8 by human PBMCs. The expected ED50 for this effect is 12-25 ng/ml. |
Results: |
N/A |
Molecular Weight: |
This protein is fused with 6 × His tag at the C-terminus, has a calculated MW of 16.7 kDa. The predicted N-terminus is Tyr 33. DTT-reduced Protein migrates as 24 kDa, 27 kDa and 31 kDa due to different glycosylation. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized powder |
Handling: |
Centrifuge the vial prior to opening. |