BioVision Products
Human CellExp™ IL-1 RII /CD121b, human recombinant
- SKU:
- 7392-50
Description
Human CellExp™ IL-1 RII /CD121b, human recombinant is available at Gentaur for Next week delivery.
Serves as a decoy receptor by competitive binding to IL1B
Biomolecule/Target: IL-1 RII /CD121b
Alternates names: CD121b, IL1RB, IL1R2, CDw121b
Synonyms: CD121b, IL1RB, IL1R2, CDw121b
Background Information: Interleukin-1 receptor type 2 (IL1R2) also known as CD121 antigen-like family member B (CDw121b), IL-1 type II receptor, Interleukin-1 receptor type II, belongs to the interleukin-1 receptor family. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R2 is non-signaling receptor for IL1A, IL1B and IL1RN, reduces IL1B activities. Serves as a decoy receptor by competitive binding to IL1B and preventing its binding to IL1R1. IL1R2 modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: IL1R2
Gene ID: 7850
NCBI Accession: P27930
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
<1 EU/μg by LAL method |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Measured by its ability to inhibit IL-1 beta dependent proliferation in D10.G4.1 mouse helper T cells. Approximately 0.5-3 µg/ml of IL1R2 will inhibit 50% of the biological response due to 50 pg/ml of recombinant human IL-1 beta. |
Results: |
Measured by its ability to inhibit IL-1 beta dependent proliferation in D10.G4.1 mouse helper T cells. Approximately 0.5-3 µg/ml of IL1R2 will inhibit 50% of the biological response due to 50 pg/ml of recombinant human IL-1 beta. |
Molecular Weight: |
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 38.6 kDa. The predicted N-terminus is Thr 14. DTT-reduced Protein migrates as 50-55 kDa due to glycosylation. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |