Human CellExp™ IFN-gamma, human recombinant

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SKU:
7271-10
$183
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Description

Human CellExp™ IFN-gamma, human recombinant is available at Gentaur for Next week delivery.

A central regulator of the immune response and signals

Biomolecule/Target: IFN-gamma

Alternates names: IFNG, IFG, IFI

Synonyms: IFNG, IFG, IFI

Background Information: Interferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFN gamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

NCBI Gene Symbol: IFN-gamma

Gene ID: 3458

NCBI Accession: P01579

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Additional Information

Size:
10 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
HEK 293 cells
Purity by SDS-PAGE:
≥93%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
< 0.1 ng/μg of protein (<1EU/μg) by LAL method
Activity (Specifications/test method):
Determined by a cytotoxicity assay using HT-29 cells. ED50 <0.05ng/ml, corresponding to a specific activity of >2X10^7 Unit/mg.
Biological activity:
Determined by a cytotoxicity assay using HT-29 cells. ED50 <0.05ng/ml, corresponding to a specific activity of >2X10^7 Unit/mg.
Results:
N/A
Molecular Weight:
Calculated MW of 16.8 kDa with no tag. The predicted N-terminus is Gln 24. DTT-reduced protein migrates as 25.0 kDa due to glycosylation.
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized powder
Handling:
Centrifuge the vial prior to opening.
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