BioVision Products
Human CellExp™ HVEM/TNFRSF14, human recombinant
- SKU:
- 7466-20
Description
Human CellExp™ HVEM/TNFRSF14, human recombinant is available at Gentaur for Next week delivery.
A type I membrane protein belonging to the TNF/NGF receptor superfamily
Biomolecule/Target: HVEM/TNFRSF14
Alternates names: TNFRSF14, ATAR, HVEA, HVEM, LIGHTR, TR2, Tumor necrosis factor receptor superfamily member 14, Herpesvirus entry mediator.
Synonyms: TNFRSF14, ATAR, HVEA, HVEM, LIGHTR, TR2, Tumor necrosis factor receptor superfamily member 14, Herpesvirus entry mediator.
Background Information: Herpesvirus entry mediator (HVEM), also known as TNFRSF14, TR2 (TNF receptor like molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LTβR, the receptor for lymphotoxin αβ heterotrimer. The role of the HVEM LIGHT /LTβ receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: TNFRSF14
Gene ID: 8764
NCBI Accession: Q92956
Additional Information
Size: |
20 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
<1 EU/μg by LAL method |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Measured by its ability to inhibit TNF-beta-mediated cytotoxicity using L929 Mouse fibrosarcoma cells. The ED50 for this effect is typically 2.5-10 μg/ml in the presence of 1 ng/ml of recombinant human TNF-beta. |
Results: |
Measured by its ability to inhibit TNF-beta-mediated cytotoxicity using L929 Mouse fibrosarcoma cells. The ED50 for this effect is typically 2.5-10 μg/ml in the presence of 1 ng/ml of recombinant human TNF-beta. |
Molecular Weight: |
This protein is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 413 amino. rhHVEM-Fc, fused with the Fc region of human IgG1 at the C-terminus and has a calculated MW of 45.4 kDa expressed. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |