BioVision Products
Human CellExp™ GHR /Growth Hormone Receptor, human recombinant
- SKU:
- 7477-10
Description
Human CellExp™ GHR /Growth Hormone Receptor, human recombinant is available at Gentaur for Next week delivery.
A receptor for pituitary gland growth hormone involved in regulating postnatal body growth
Biomolecule/Target: GHR /Growth Hormone Receptor
Alternates names: GHR, GHBP, GH receptor
Synonyms: GHR, GHBP, GH receptor
Background Information: Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: GHR
Gene ID: 2690
NCBI Accession: P10912
Additional Information
Size: |
10 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
<1 EU/μg by LAL method |
Activity (Specifications/test method): |
N/A |
Biological activity: |
The bioactivity is measured by its binding ability to human GH in a SPR assay. Covalently immobilized Recombinant Human GHR Protein (Cat# GHR-H5222) can bind human growth hormone protein with affinity constant of 1.28 ±0.16 nM range. |
Results: |
The bioactivity is measured by its binding ability to human GH in a SPR assay. Covalently immobilized Recombinant Human GHR Protein (Cat# GHR-H5222) can bind human growth hormone protein with affinity constant of 1.28 ±0.16 nM range. |
Molecular Weight: |
This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 28.5 kDa. The predicted N-terminus is Ala 27. DTT-reduced Protein migrates as 40-50 kDa in SDS-PAGE due to glycosylation. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |