BioVision Products
Human CellExp™ EPOR/Erythropoietin Receptor, human recombinant
- SKU:
- 7454-50
Description
Human CellExp™ EPOR/Erythropoietin Receptor, human recombinant is available at Gentaur for Next week delivery.
A major glycoprotein hormone regulator of mammalian erythropoiesis
Biomolecule/Target: EPOR/Erythropoietin Receptor
Alternates names: EPOR, Erythropoietin Receptor.
Synonyms: EPOR, Erythropoietin Receptor.
Background Information: Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR is a type 1 single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR pre-exists as dimers which upon binding of a 34 kDa ligand erythropoietin (EPO), changes its homodimerized state. These conformational changes result in the autophosphorylation of Jak2 kinases that are pre-associated with the receptor. Erythropoietin is necessary to maintain endothelial cells and to promote tumor angiogenesis, hence the dysregulation of EpoR may affect the growth of certain tumors. EpoR signaling prevents neuronal death and ischemic injury.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: EPOR
Gene ID: 2057
NCBI Accession: P19235
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥92% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
<1 EU/μg by LAL method |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Measured by its ability to inhibit Epo-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is typically 2 - 6 ng/ml in the presence of 0.2 U/ml of rhEpo-Fc. |
Results: |
Measured by its ability to inhibit Epo-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is typically 2 - 6 ng/ml in the presence of 0.2 U/ml of rhEpo-Fc. |
Molecular Weight: |
This protein is fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 50.1 kDa. The predicted N-terminus is Ala 25. DTT-reduced Protein migrates as 55-60 kDa due to glycosylation. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |