Human CellExp™ GCSFR /CD114, human recombinant

(No reviews yet) Write a Review
SKU:
7480-20
$183
Frequently bought together:

Description

Human CellExp™ GCSFR /CD114, human recombinant is available at Gentaur for Next week delivery.

Initiates cell proliferation and differentiation into mature neutrophilic granulocytes and macrophages

Biomolecule/Target: GCSFR /CD114

Alternates names: CSF3R, CD114, GCSFR

Synonyms: CSF3R, CD114, GCSFR

Background Information: Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as Cluster of Differentiation 114 (CD114), CSF3R and GCSF, is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF), a cytokine that plays a critical role in the regulation of the activation, proliferation, differentiation, and survival of the neutrophilic granulocyte lineage. G-CSFR belongs to a family of cytokine receptors known as the hematopoietin receptor family. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type I?II (FNIII) domains in the extracellular region. G-CSFR is present mainly on precursor cells in the bone marrow, and, in response to stimulation by G-CSF, initiates cell proliferation and differentiation into mature neutrophilic granulocytes and macrophages. G-CSFR mediates the specific effect of GCSF through activating a variety of intracellular signaling cascades, including the Jak/Stat, PI3/Akt, Ras-Raf-MAP kinase, and Src family kinase pathways, and thus functions in defense against infection, inflammation and repair, and in the maintenance of steady state hematopoiesis. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. Mutations in the intracellular part of this receptor are also associated with certain types of leukemia.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

NCBI Gene Symbol: GCSFR

Gene ID: 1441

NCBI Accession: Q99062

View AllClose

Additional Information

Size:
20 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
HEK293 cells
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
<1 EU/μg by LAL method
Activity (Specifications/test method):
N/A
Biological activity:
Measured by its ability to inhibit the GCSF-induced proliferation of NFS-60 mouse myeloid cells. The ED50 for this effect is typically 0.02-2 μg /ml in the presence of 0.125 ng /ml of recombinant human GCSF.
Results:
Measured by its ability to inhibit the GCSF-induced proliferation of NFS-60 mouse myeloid cells. The ED50 for this effect is typically 0.02-2 μg /ml in the presence of 0.125 ng /ml of recombinant human GCSF.
Molecular Weight:
This protein is fused with 6×his tag at the N-terminus, has a calculated MW of 69 kDa expressed. The predicted N-terminus is Glu 25. Protein migrates as 94 kDa in reduced SDS-PAGE resulting from glycosylation.
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized
Handling:
Centrifuge the vial prior to opening.
View AllClose

0 Reviews

View AllClose