Human CellExp™ Furin, human recombinant

(No reviews yet) Write a Review
SKU:
7249-10
$719.07
Frequently bought together:

Description

Human CellExp™ Furin, human recombinant is available at Gentaur for Next week delivery.

Involved in processing of latent precursor proteins into their biologically active products

Biomolecule/Target: Furin

Alternates names: FURIN, FUR, PACE, PCSK3, SPC1

Synonyms: FURIN, FUR, PACE, PCSK3, SPC1

Background Information: Furin also known as paired basic Amino acid Cleaving Enzyme (PACE), is an enzyme which belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. The expression of furin in T-cells is required for maintenance of peripheral immune tolerance. Furin cleaves proteins just downstream of a basic amino acid target sequence (canonically, Arg-X-(Arg/Lys) -Arg'). PACE is a calcium-dependent serine endoprotease that can efficiently cleave precursor proteins at their paired basic amino acid processing sites. In addition to processing cellular precursor proteins, furin is also utilized by a number of pathogens. For example, the envelope proteins of viruses such as HIV, influenza and dengue fever viruses must be cleaved by furin or furin-like proteases to become fully functional. PACE also play a role in tumor progression.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

NCBI Gene Symbol: PACE

Gene ID: 5045

NCBI Accession: P09958

View AllClose

Additional Information

Size:
10 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
HEK 293 cells
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
< 0.1 ng/μg of protein (<1EU/μg).
Activity (Specifications/test method):
Measured by its ability to cleave the fluorogenic peptide substrate p – Glu – Arg – Thr – Lys – Arg – AMC. The bioactivity was measured in 100 μL reaction mixture containing 4 µg/ml of rhFurin, 50 μM substrate, 25 mM Tris, 1 mM CaCl2, 0.5% (w/v) Brij
Biological activity:
Measured by its ability to cleave the fluorogenic peptide substrate p – Glu – Arg – Thr – Lys – Arg – AMC. The bioactivity was measured in 100 μL reaction mixture containing 4 µg/ml of rhFurin, 50 μM substrate, 25 mM Tris, 1 mM CaCl2, 0.5% (w/v) Brij
Results:
N/A
Molecular Weight:
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 66.5 kDa. The predicted N-terminus is Asp 108. DTT-reduced Protein migrates as 55-66 kDa.
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized powder
Handling:
Centrifuge the vial prior to opening.
View AllClose

0 Reviews

View AllClose