BioVision Products
Human CellExp™ FCER1A /FCE1A, human recombinant
- SKU:
- 7473-50
Description
Human CellExp™ FCER1A /FCE1A, human recombinant is available at Gentaur for Next week delivery.
Plays a central role in allergic response
Biomolecule/Target: FCER1A /FCE1A
Alternates names: FCER1A, FCE1A, Ferris
Synonyms: FCER1A, FCE1A, Ferris
Background Information: High affinity immunoglobulin epsilon receptor subunit alpha (FCER1A) is also known as Fc-epsilon RI-alpha (FcERI), IgE Fc receptor subunit alpha, FCE1A. FCER1A contains two Ig-like (immunoglobulin-like) domains. FCER1A binds to the Fc region of immunoglobulins epsilon and is a high affinity receptor. FCER1A is responsible for initiating the allergic response, which binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. FCER1A plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: FCER1A
Gene ID: 2205
NCBI Accession: P12319
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
<1 EU/μg by LAL method |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Measured by its binding ability in a functional ELISA. Immobilized human FCER1A at 2 μg/ml (100 μl/well). The concentration of Human IgE that produces 50% of the optimal binding response is found to be approximately 0.1 - 0.4 µg/ml. |
Results: |
Measured by its binding ability in a functional ELISA. Immobilized human FCER1A at 2 μg/ml (100 μl/well). The concentration of Human IgE that produces 50% of the optimal binding response is found to be approximately 0.1 - 0.4 µg/ml. |
Molecular Weight: |
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 21.9 kDa. The predicted N-terminus is Val 26. DTT-reduced Protein migrates as 40-60 kDa due to glycosylation. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |