BioVision Products
Human CellExp™ CD23, human recombinant
- SKU:
- 7435-50
Description
Human CellExp™ CD23, human recombinant is available at Gentaur for Next week delivery.
Mediates transportation during antibody feedback regulation.
Biomolecule/Target: CD23
Alternates names: FCER2; FCER-2; CD23; CD-23; CD23A; CD-23A; CLEC4J; FCE2; FCE-2; IGEBF; FcεRII; FcεRⅡ; Fc epsilon RII
Synonyms: FCER2; FCER-2; CD23; CD-23; CD23A; CD-23A; CLEC4J; FCE2; FCE-2; IGEBF; FcεRII; FcεRⅡ; Fc epsilon RII
Background Information: Cluster of differentiation 23 (CD23), also known as Low affinity immunoglobulin epsilon Fc receptor (FCER2), C-type lectin domain family 4 member J (CLEC4J), Fc-epsilon-RII (FcεRII), Immunoglobulin E-binding factor (IGEBF), is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. Unlike many of the antibody receptors, CD23 is a C-type lectin. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. CD23 is known to have role of transportation in antibody feedback regulation. Antigen that enters the blood stream is captured by antigen specific IgE antibodies. The IgE immune complexes that are formed bind to CD23 molecules on B cells, and are transported to the B cell follicles of the spleen. The antigen is then transferred from CD23+ B cells to CD11c+ antigen presenting cells. The CD11c+ cells in turn present the antigen to CD4+ T cells, which can lead to an enhanced antibody response. In flow cytometry, CD23 is helpful in the differentiation of chronic lymphocytic leukemia (CD23-positive) from mantle cell leukemia (CD23-negative).
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: FCER2
Gene ID: 2208
NCBI Accession: P06734
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
<1 EU/μg by LAL method |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Measured by its binding ability in a functional ELISA. Immobilized rhCD23 at 2 μg/ml (100 μl/well) can bind human IgE with a linear range of 0.01-1.2 μg/ml. |
Results: |
Measured by its binding ability in a functional ELISA. Immobilized rhCD23 at 2 μg/ml (100 μl/well) can bind human IgE with a linear range of 0.01-1.2 μg/ml. |
Molecular Weight: |
This protein is fused with a fused with 6×his tag at the N-terminus and has a calculated MW of 33.2 kDa expressed. The predicted N-terminus is Asp48. Protein migrates as 40-44 kDa in reduced SDS-PAGE resulting from glycosylation. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |