BioVision Proteins
Human CellExp™ Biotinylated PD-1 / PDCD1, His Tag & Fc Tag, human recombinant
- SKU:
- 7877-5
Description
Human CellExp™ Biotinylated PD-1 / PDCD1, His Tag & Fc Tag, human recombinant is available at Gentaur for Next week delivery.
Inhibits the T-cell proliferation and production of IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway.
Biomolecule/Target: PD-1/PDCD1
Alternates names: PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l
Synonyms: PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l
Background Information: Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. The Biotinylated Human PD-1 / PDCD1 Protein, His Tag & Fc Tag was produced using proprietary single-point enzymatic biotinlabeling technology at C-terminus to retain its native conformational structure and, therefore, bioactivity, and has been used successfully for high sensitivity and low variability assays, such as ELISA, western blotting, inhibition assay, pull-down, SPR, FACS, and affinity purification, together with anti-biotin antibody, streptavidin or avidin-conjugated probes.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: PDCD1
Gene ID: 5133
NCBI Accession: Q15116
Additional Information
Size: |
5 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
<1 EU/μg by LAL method |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, Fc Tag at 5 μg/mL (100 µl/well), can bind Biotinylated Human PD-1, His Tag & Fc Tag with a linear range of 0.01-0.1 μg/mL. |
Results: |
Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, Fc Tag at 5 μg/mL (100 µl/well), can bind Biotinylated Human PD-1, His Tag & Fc Tag with a linear range of 0.01-0.1 μg/mL. |
Molecular Weight: |
Biotinylated rhPDCD1 Fc Chimera, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 42.6 kDa. The predicted N-terminus is Pro 21. DTT-reduced Protein migrates as 50 kDa due to glycosylation. The primary amine of biotinylat |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |