Human CellExp™ Biotinylated PD-1 / PDCD1, His Tag & Fc Tag, human recombinant

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SKU:
7877-5
€185
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Description

Human CellExp™ Biotinylated PD-1 / PDCD1, His Tag & Fc Tag, human recombinant is available at Gentaur for Next week delivery.

Inhibits the T-cell proliferation and production of IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway.

Biomolecule/Target: PD-1/PDCD1

Alternates names: PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l

Synonyms: PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l

Background Information: Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. The Biotinylated Human PD-1 / PDCD1 Protein, His Tag & Fc Tag was produced using proprietary single-point enzymatic biotinlabeling technology at C-terminus to retain its native conformational structure and, therefore, bioactivity, and has been used successfully for high sensitivity and low variability assays, such as ELISA, western blotting, inhibition assay, pull-down, SPR, FACS, and affinity purification, together with anti-biotin antibody, streptavidin or avidin-conjugated probes.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

NCBI Gene Symbol: PDCD1

Gene ID: 5133

NCBI Accession: Q15116

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Additional Information

Size:
5 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
HEK293 cells
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
<1 EU/μg by LAL method
Activity (Specifications/test method):
N/A
Biological activity:
Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, Fc Tag at 5 μg/mL (100 µl/well), can bind Biotinylated Human PD-1, His Tag & Fc Tag with a linear range of 0.01-0.1 μg/mL.
Results:
Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, Fc Tag at 5 μg/mL (100 µl/well), can bind Biotinylated Human PD-1, His Tag & Fc Tag with a linear range of 0.01-0.1 μg/mL.
Molecular Weight:
Biotinylated rhPDCD1 Fc Chimera, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 42.6 kDa. The predicted N-terminus is Pro 21. DTT-reduced Protein migrates as 50 kDa due to glycosylation. The primary amine of biotinylat
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized
Handling:
Centrifuge the vial prior to opening.
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