HPRT1, human recombinant

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SKU:
P1092-50
$183
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Description

HPRT1, human recombinant is available at Gentaur for Next week delivery.

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate.

Biomolecule/Target: HPRT1

Alternates names: Hypoxanthine-guanine phosphoribosyltransferase, GPRT, HGPRTase, HPRT

Synonyms: Hypoxanthine-guanine phosphoribosyltransferase, GPRT, HGPRTase, HPRT

Background Information: Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP. Recombinant human HPRT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Reconstitution Instructions: N/A

NCBI Gene Symbol: HPRT1

Gene ID: 3251

NCBI Accession: P00492

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Additional Information

Size:
50 µg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
E. Coli
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
N/A
Activity (Specifications/test method):
Measured by the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.
Biological activity:
>15 units/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.
Results:
>15 units/mg
Molecular Weight:
26.7 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
0.5 mg/ml
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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