Description
HPRT1, human recombinant is available at Gentaur for Next week delivery.
Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate.
Biomolecule/Target: HPRT1
Alternates names: Hypoxanthine-guanine phosphoribosyltransferase, GPRT, HGPRTase, HPRT
Synonyms: Hypoxanthine-guanine phosphoribosyltransferase, GPRT, HGPRTase, HPRT
Background Information: Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP. Recombinant human HPRT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: HPRT1
Gene ID: 3251
NCBI Accession: P00492
Additional Information
Size: |
50 µg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. Coli |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Measured by the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C. |
Biological activity: |
>15 units/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C. |
Results: |
>15 units/mg |
Molecular Weight: |
26.7 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
0.5 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |