BioVision Proteins
Guanylate kinase, human recombinant
- SKU:
- P1101-10
Description
Guanylate kinase, human recombinant is available at Gentaur for Next week delivery.
Essential for recycling GMP and indirectly, cGMP.
Biomolecule/Target: GUK1
Alternates names: GMK, GMP kinase, Guanylate kinase
Synonyms: GMK, GMP kinase, Guanylate kinase
Background Information: GUK1, also known as GMK, belongs to the guanylate kinase family. This protein exists as a monomer that catalyzes the ATP-dependent conversion of GMP to GDP, thereby playing an essential role in the recycling of GMP. Via its catalytic activity, GUK1 is thought to participate in regulating the supply of guanine nucleotides to signal transduction pathways. Overexpression of GUK1 is associated with pituitary adenocarcinomas, suggesting that GUK1 is involved in tumorigenesis. Recombinant human GUK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: GUK1
Gene ID: 2987
NCBI Accession: Q16774
Additional Information
Size: |
10 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. Coli |
Purity by SDS-PAGE: |
≥90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Measured by its ability to convert 1.0 umole of GMP and ATP to GDP and ADP per minute at pH 7.5 at 37C in coupled system with PK/LDH. |
Biological activity: |
Specific activity is >100 units/mg and is defined as the amount of enzyme that convert 1.0 umole of GMP and ATP to GDP and ADP per minute at pH 7.5 at 37C in coupled system with PK/LDH |
Results: |
>100 units/mg |
Molecular Weight: |
23.9 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
1 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |