Description
Glutamate Decarboxylase α (GAD α), E. coli Recombinant is available at Gentaur for Next week delivery.
Converts glutamate to gamma-aminobutyrate (GABA)
Biomolecule/Target:
Alternates names: GAD-alpha, gadA, gadS
Synonyms: GAD-alpha, gadA, gadS
Background Information: Glutamate Decarboxylase α is a pyridoxal 5′-phosphate dependent enzyme. GAD α catalyzes conversion of glutamate to GABA. Aberrant expressions of GAD α have been associated with diabetes and neurodegenerative diseases such as Alzheimers and Parkinson’s Disease.
Reconstitution Instructions: Reconstitute the lyophilized protein in 10% glycerol with 5 mM 2-Mercaptoethanol to a final concentration of 1.0 mU/ml and incubate the reconstituted protein at 25 °C for 15 minutes.
NCBI Gene Symbol: gadA
Gene ID: 948027
NCBI Accession: P69908
Additional Information
Size: |
50 µU |
Adwords: |
False |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
E Coli |
Recombinant: |
True |
Source: |
E. coli |
Purity by SDS-PAGE: |
>95% |
Assay: |
SDS-PAGE |
Activity (Specifications/test method): |
This enzyme has a specific activity of ≥ 1 mU/mg based on its ability to convert glutamate to GABA, which can be detected fluorometrically at Ex/Em=535/595. |
Results: |
1 mU/mg |
Molecular Weight: |
53.6 kDa with C-terminal 6X-His tag |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Appearance: |
Lyophilized powder |
Handling: |
Centrifuge the vial prior to opening. |