Homocysteine α,γ-Lyase (rHCYase), Active, Recombinant

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SKU:
P1117-200
€185
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Description

Homocysteine α,γ-Lyase (rHCYase), Active, Recombinant is available at Gentaur for Next week delivery.

Enzyme that can metabolize homocysteine into α-keto butyrate, hydrogen sulfide and ammonia.

Biomolecule/Target: mgl1

Alternates names: α,γ-Homocysteinase, Methionine gamma-lyase, mgl1

Synonyms: Homocysteine α,γ-Lyase (rHCYase)

Background Information: Recombinant Homocysteine α,γ-lyase is a pyridoxal-5’-phosphate dependent enzyme. It is a mutant of homocysteinase from Trichomonas vaginalis encoded by mgl1 gene, containing three point mutations, such as; Phe47Leu, Asp172Glu, Ser308Tyr. Its native molecular mass is 170 kD, with four seemingly identical subunits, each with molecular mass of 43-45 kD. The enzyme can metabolize homocysteine into α-keto butyrate, hydrogen sulfide and ammonia.

Reconstitution Instructions: Reconstitute in 40 mM Sodium phosphate buffer, pH 8.1.

NCBI Gene Symbol: mgl1

Gene ID:

NCBI Accession: O15564

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Additional Information

Size:
200 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Trichomonas vaginalis
Recombinant:
Yes
Source:
E. Coli
Purity by SDS-PAGE:
> 95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
N/A
Activity (Specifications/test method):
Measured by its ability to convert 1 µmole of homocysteine into hydrogen sulfide, per minute at 25 ºC and pH 8.1 in the presence of pyridoxal phosphate
Biological activity:
Specific activity is ≥ 5 mU/mg in which one Unit enzyme converts 1 µmole of homocysteine into hydrogen sulfide, per minute at 25 ºC and pH 8.1 in the presence of pyridoxal phosphate
Results:
≥ 5 mU/mg
Molecular Weight:
43 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized
Handling:
Centrifuge the vial prior to opening.
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