Glucokinase, human pancreatic, recombinant

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SKU:
7777-10
€185
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Description

Glucokinase, human pancreatic, recombinant is available at Gentaur for Next week delivery.

Plays an important role in the regulation of carbohydrate metabolism

Biomolecule/Target:

Alternates names: Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D

Synonyms:

Background Information: Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase (Accession# NP_000153)) has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.

Reconstitution Instructions: N/A

NCBI Gene Symbol: GCK

Gene ID: 2645

NCBI Accession: P35557

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Additional Information

Size:
10 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human pancreas
Recombinant:
Yes
Source:
E. coli.
Purity by SDS-PAGE:
≥80%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
N/A
Activity (Specifications/test method):
303 pmol/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30oC. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose
Biological activity:
N/A
Results:
N/A
Molecular Weight:
53.1 kDa
Storage Temperature:
-80°C
Shelf Life:
12 months
Concentration:
39.3 mg/ml
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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