BioVision Proteins
Glucokinase, human pancreatic, recombinant
- SKU:
- 7777-10
Description
Glucokinase, human pancreatic, recombinant is available at Gentaur for Next week delivery.
Plays an important role in the regulation of carbohydrate metabolism
Biomolecule/Target:
Alternates names: Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D
Synonyms:
Background Information: Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase (Accession# NP_000153)) has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.
Reconstitution Instructions: N/A
NCBI Gene Symbol: GCK
Gene ID: 2645
NCBI Accession: P35557
Additional Information
Size: |
10 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human pancreas |
Recombinant: |
Yes |
Source: |
E. coli. |
Purity by SDS-PAGE: |
≥80% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
303 pmol/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30oC. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose |
Biological activity: |
N/A |
Results: |
N/A |
Molecular Weight: |
53.1 kDa |
Storage Temperature: |
-80°C |
Shelf Life: |
12 months |
Concentration: |
39.3 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |