Description
GAPDH, Active, human recombinant is available at Gentaur for Next week delivery.
Involved in glycolysis
Biomolecule/Target: GAPDH
Alternates names: Glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD
Synonyms: Glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD
Background Information: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-kDa subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recombinant GAPDH protein was expressed in E.coli and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: Gapdh
Gene ID: 2597
NCBI Accession: P04406
Additional Information
Size: |
50 µg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. Coli |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Measured by its ability to convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C |
Biological activity: |
Specific activity is > 90 units/mg, and is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C. |
Results: |
> 90 units/mg |
Molecular Weight: |
36 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
1 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |