GalNAc-T3, soluble fragment, Human Recombinant

(No reviews yet) Write a Review
SKU:
P1216-5
£135.37
Frequently bought together:

Description

GalNAc-T3, soluble fragment, Human Recombinant is available at Gentaur for Next week delivery.

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis

Biomolecule/Target: GALNT3

Alternates names: Polypeptide N-acetylgalactosaminyltransferase 3, Polypeptide GalNAc transferase 3, GaNTase 3

Synonyms: Polypeptide N-acetylgalactosaminyltransferase 3, Polypeptide GalNAc transferase 3, GaNTase 3

Background Information: Polypeptide N-acetylgalactosaminyltransferase 3 (GalNAc-T3) catalyzes the transfer of N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the hydroxyl group of serine and threonine residues. Twenty GalNAc-T genes have been identified in humans and most have been shown to represent active GalNAc-Ts. All isoforms are type II transmembrane proteins, with different but partly overlapping substrate preferences. The GalNAc-Ts control the initiation of mucin-type O-linked glycosylation and determine the location and density of O-glycans in a protein. Addition of GalNAc to an unglycosylated Ser/Thr residue creates the Tn antigen GalNAcα1-S/T, and subsequent addition of sialic acid by ST6GalNAc-I forms the cancer associated STn antigen. GalNAc-T3 is highly expressed in pancreas and testis, and weakly expressed in a few other organs such as placenta. The expression levels of GalNAc-T3 were found to be upregulated in highly differentiated types of adenocarcinoma cell lines as opposed to less differentiated types. Overexpression of GalNAc-T3 has also been found to promote pancreatic cancer cell growth. Mutations in GalNAc-T3 were found to be involved in familial tumoral calcinosis, which may be mediated through glycosylation of fibroblast growth factor 23, protecting it from proteolytic cleavage. GalNAc-T3 has a preference for mono- or diglycosylated substrates.

Reconstitution Instructions: N/A

NCBI Gene Symbol: GALNT3

Gene ID: 2591

NCBI Accession: Q14435

View AllClose

Additional Information

Size:
5 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
Insect cells
Purity by SDS-PAGE:
> 95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
N/A
Activity (Specifications/test method):
Measured by transfer of N-acetylgalatosamine (GalNac) from UDP-Gal to the peptide EA2, as measured with a phosphatase-coupled assay.
Biological activity:
Specific activity >500 pmol/min/ug
Results:
>500 pmol/min/ug
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
466 ug/ml
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
View AllClose

0 Reviews

View AllClose