Description
FGF-2/FGF-basic, bovine recombinant is available at Gentaur for Next week delivery.
A growth factor involved in angiogenesis, wound healing and embryonic development
Biomolecule/Target: FGF-2
Alternates names: FGF2, HBGF-2, Prostatropin, Basic fibroblast growth factor, Heparin-binding growth factor 2
Synonyms: FGF-2, FGF2
Background Information: FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. FGF-2 Bovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17250 Dalton. The Fibroblast Growth Factor 2 is purified by proprietary chromatographic techniques. The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser.
Reconstitution Instructions: Reconstitute in sterile water to a concentration not less than 0.1 mg/ml. Aliquot and store at –20°C. Avoid freeze-thaw cycles.
NCBI Gene Symbol: FGF-2
Gene ID: 281161
NCBI Accession: P03969
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Bovine |
Recombinant: |
Yes |
Source: |
E. coli |
Purity by SDS-PAGE: |
≥97% |
Assay: |
SDS-PAGE |
Purity: |
≥97% |
Assay 2: |
HPLC |
Endotoxin Level: |
<0.1 ng/μg |
Activity (Specifications/test method): |
N/A |
Biological activity: |
The ED50, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be <0.1 ng/ml, corresponding to a specific activity of 10 MUnits/mg. |
Results: |
<0.1 ng/ml |
Molecular Weight: |
17.25 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized protein |
Handling: |
Centrifuge the vial prior to opening. |