Carboxylesterase 1D, mouse recombinant

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SKU:
P1094-2
$719.07
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Description

Carboxylesterase 1D, mouse recombinant is available at Gentaur for Next week delivery.

Protein involved in the metabolism of xenobiotics and of natural substrates

Biomolecule/Target: Ces1d

Alternates names: Ces1d, Carboxylesterase 3, FAEE synthase, TGH, Ces3

Synonyms: Ces1d, Carboxylesterase 3, FAEE synthase, TGH, Ces3

Background Information: Ces1d, also known as carboxylesterase 1D, is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. It is the principle lipase of white adipose tissue fat cake extracts. Partially purified white adipose tissue Ces1d had lipase activity as well as lesser but detectable neutral cholesteryl ester hydrolase activity. The protein shows low catalytic efficiency for hydrolysis of CPT-11, a prodrugs for camptothecin used in cancer therapeutics. Recombinant mouse Ces1d, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Reconstitution Instructions: N/A

NCBI Gene Symbol: Ces1d

Gene ID: 104158

NCBI Accession: Q8VCT4

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Additional Information

Size:
2 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Mouse
Recombinant:
Yes
Source:
Insect cell (Baculovirus)
Purity by SDS-PAGE:
≥90%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
< 1 EU/μg
Activity (Specifications/test method):
Measured by the hydrolysis of 1pmole of p-nitrophenyl acetate to pnitrophenol per minute at pH 7.5 at 37C.
Biological activity:
> 80,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to pnitrophenol per minute at pH 7.5 at 37C.
Results:
> 80,000 pmol/min/ug
Molecular Weight:
60.9 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
0.5 mg/ml
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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