BioVision Proteins
Carboxylesterase 1D, mouse recombinant
- SKU:
- P1094-2
Description
Carboxylesterase 1D, mouse recombinant is available at Gentaur for Next week delivery.
Protein involved in the metabolism of xenobiotics and of natural substrates
Biomolecule/Target: Ces1d
Alternates names: Ces1d, Carboxylesterase 3, FAEE synthase, TGH, Ces3
Synonyms: Ces1d, Carboxylesterase 3, FAEE synthase, TGH, Ces3
Background Information: Ces1d, also known as carboxylesterase 1D, is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. It is the principle lipase of white adipose tissue fat cake extracts. Partially purified white adipose tissue Ces1d had lipase activity as well as lesser but detectable neutral cholesteryl ester hydrolase activity. The protein shows low catalytic efficiency for hydrolysis of CPT-11, a prodrugs for camptothecin used in cancer therapeutics. Recombinant mouse Ces1d, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: Ces1d
Gene ID: 104158
NCBI Accession: Q8VCT4
Additional Information
Size: |
2 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Mouse |
Recombinant: |
Yes |
Source: |
Insect cell (Baculovirus) |
Purity by SDS-PAGE: |
≥90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
< 1 EU/μg |
Activity (Specifications/test method): |
Measured by the hydrolysis of 1pmole of p-nitrophenyl acetate to pnitrophenol per minute at pH 7.5 at 37C. |
Biological activity: |
> 80,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to pnitrophenol per minute at pH 7.5 at 37C. |
Results: |
> 80,000 pmol/min/ug |
Molecular Weight: |
60.9 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
0.5 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |
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