Description
GAPDH, mouse recombinant is available at Gentaur for Next week delivery.
Involved in glycolysis
Biomolecule/Target: GAPDH
Alternates names: G3PD, GAPD, HEL-S-162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2
Synonyms: G3PD, GAPD, HEL-S-162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2
Background Information: Gapdh, also known as glyceraldehyde 3-phosphate dehydrogenase, is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, Gapdh has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. Recombinant mouse Gapdh, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: Gapdh
Gene ID: 14433
NCBI Accession: P16858
Additional Information
Size: |
50 µg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Mouse |
Recombinant: |
Yes |
Source: |
E. Coli |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Measured by its ability to convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C. |
Biological activity: |
Specific activity is > 40 units/mg, and is defined as theamount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C. |
Results: |
> 40 units/mg |
Molecular Weight: |
38.2kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
0.25 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |