C5a (His-Tag), murine recombinant

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SKU:
4895A-20
$183
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Description

C5a (His-Tag), murine recombinant is available at Gentaur for Next week delivery.

An anaphylatoxin

Biomolecule/Target:

Alternates names: Complement Fragments C5a, C5a anaphylatoxin chemotactic receptor

Synonyms: Complement Fragments C5a

Background Information: Mouse complement C5a is a 77-amino acid peptide generated during complement activation from the α-chain of complement C5. Mouse C5a shares 60% and 82% amino acid sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells. The 9 kDa mouse C5a protein expressed in E. coli contains a N-terminal His-Tag sequence.

Reconstitution Instructions: Reconstitute to a concentration of 1.0 mg/ml in ice-cold H₂O containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C.

NCBI Gene Symbol: C5a

Gene ID: 15139

NCBI Accession: NP_034536

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Additional Information

Size:
20 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Mouse
Recombinant:
Yes
Source:
E. coli
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
<0.1 ng/μg
Activity (Specifications/test method):
N/A
Biological activity:
ED₅₀ 1-3 nM as determined by enzyme release assay of myeloperoxidase
Results:
1-3 nmolar
Molecular Weight:
9 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized protein
Handling:
Centrifuge the vial prior to opening.
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