Description
C5a (His-Tag), murine recombinant is available at Gentaur for Next week delivery.
An anaphylatoxin
Biomolecule/Target:
Alternates names: Complement Fragments C5a, C5a anaphylatoxin chemotactic receptor
Synonyms: Complement Fragments C5a
Background Information: Mouse complement C5a is a 77-amino acid peptide generated during complement activation from the α-chain of complement C5. Mouse C5a shares 60% and 82% amino acid sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells. The 9 kDa mouse C5a protein expressed in E. coli contains a N-terminal His-Tag sequence.
Reconstitution Instructions: Reconstitute to a concentration of 1.0 mg/ml in ice-cold H₂O containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C.
NCBI Gene Symbol: C5a
Gene ID: 15139
NCBI Accession: NP_034536
Additional Information
Size: |
20 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Mouse |
Recombinant: |
Yes |
Source: |
E. coli |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
<0.1 ng/μg |
Activity (Specifications/test method): |
N/A |
Biological activity: |
ED₅₀ 1-3 nM as determined by enzyme release assay of myeloperoxidase |
Results: |
1-3 nmolar |
Molecular Weight: |
9 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized protein |
Handling: |
Centrifuge the vial prior to opening. |