C5a (His-Tag), human recombinant

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SKU:
4995A-20
$183
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Description

C5a (His-Tag), human recombinant is available at Gentaur for Next week delivery.

A potent chemotactic factor for human peripheral blood neutrophils and monocytes

Biomolecule/Target:

Alternates names: C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin chemotactic receptor (C5a-R), (C5aR,) (CD antigen CD88), C5AR1, C5AR, C5R1

Synonyms: Recombinant human C5a (His-Tag)

Background Information: Human complement C5a is a 74-amino acid glycopeptide generated during complement activation from the a-chain of complement C5. C5a is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. The 74 amino acid, human C5a protein expressed in E. coli with a 6 amino acid, N-terminal His·Tag sequence and 28 amino acid proprietary vector sequence necessary for expression (C5a without His-tag is alailable, BioVision Cat # 4995B). The recombinant protein is not further processed to remove the additional sequence. Recombinant C5a has an activity similar to that of serum derived C5a based on enzyme assay of myeloperoxidase release.

Reconstitution Instructions: Reconstitute to a concentration of 1.0 mg/ml in ice-cold H₂O containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C for future use. Frozen/thaw will result in some loss of activity.

NCBI Gene Symbol:

Gene ID:

NCBI Accession:

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Additional Information

Size:
20 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Recombinant:
Yes
Source:
E. coli
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
<0.1 ng/μg
Activity (Specifications/test method):
N/A
Biological activity:
Human recombinant C5a is as active as that of serum derived C5a based on enzyme release assay of myeloperoxidase. The current lot of ED₅₀ = 1.36 nmolar.
Results:
N/A
Molecular Weight:
(74 amino acids)
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized protein
Handling:
Centrifuge the vial prior to opening.
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