Argininosuccinate Synthetase (ASS1), E. coli, Recombinant

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SKU:
P1412-50
€185
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Description

Argininosuccinate Synthetase (ASS1), E. coli, Recombinant is available at Gentaur for Next week delivery.

Involved in urea cycle that catalyzes aspartate, citrulline, and ATP to form argininosuccinate

Biomolecule/Target:

Alternates names: ASSCTLN1, Citrulline--aspartate ligase, argG,, argininosuccinate synthase 1

Synonyms: ASSCTLN1, Citrulline--aspartate ligase, argG,, argininosuccinate synthase 1

Background Information: Argininosuccinate Synthetase is part of the urea cycle. It catalyzes aspartate, citrulline, and ATP to form argininosuccinate. Mutations in the enzyme cause Citrullinemia, preventing the liver from processing nitrogen to urea. Lack of argininosuccinate synthase has also been observed in pancreatic cancer, liver cancer, melanoma

Reconstitution Instructions: Reconstitute the lyophilized protein in H2O with concentration of 1.0 mU/ml. Incubate the reconstituted protein at 25 °C for 15 minutes.

NCBI Gene Symbol: argG

Gene ID: 947590

NCBI Accession: P0A6E4

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Additional Information

Size:
50 mU
Adwords:
False
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
E Coli
Recombinant:
True
Source:
E.coli
Purity by SDS-PAGE:
>95%
Assay:
SDS-PAGE.
Activity (Specifications/test method):
This enzyme has a specific activity of ≥ 500 mU/mg based on its ability to convert citrulline to argininosuccinate, which can be detected fluorometrically at Ex/Em = 535/595 using BioVision’s Methionine Assay Kit (K442).
Results:
≥ 500 mU/mg
Molecular Weight:
50 kDa with N-terminal 6X-His tag
Storage Temperature:
-20°C
Shelf Life:
12 months
Appearance:
Lyophilized powder
Handling:
Centrifuge the vial prior to opening.
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