Rabbit anti-EBOV Soluble GP (sGP) pAb

Rabbit anti-EBOV Soluble GP (sGP) pAb is available at Gentaur for Next Week Delivery.

Product Type: polyclonal antibody

Host: Rabbit

Clonality: polyclonal

Applications: ELISA, WB

Immunogen: Peptide sequence to Ebola Virus (EBOV) soluble glycoprotein (sGP) (cat 0565-001).

Description: Affinity purified rabbit polyclonal antibody reactive to EBOV sGP. The antibody detects EBOV sGP in Western blot and ELISA.

Supplied: 100 µg of antibody is supplied in PBS + 0.02% sodium azide at a concentration of 0.384
mg/mL.

Purification: Antibody is affinity purified using immobilized immunogen.

Relevance: The antibody can be used for detection of EBOV sGP.

Recommended Dilutions:
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution. Internal QC demonstrates good detection of EBOV sGP when
using antibody at 0.1 µg/mL.

Storage: 2-3 weeks +4°C, -20°C long term

Related Products: IBT provides a wide array of antifilovirus specific antibodies and other infectious disease
reagents. Please see our website, www.ibtbioservices.com
for more details.

Western Blot Data:

Western blots were detected under (A) reduced
and denatured conditions and (B) non-reduced and
denatured conditions with rabbit anti-EBOV sGP
antibody at 0.1 µg/mL and visualized using an antirabbit                                                                                                IgG-HRP conjugate and TMB Membrane
substrate. EBOV sGP is visualized at 200 ng, 100
ng, and 50 ng (lane 1, 2, and 3 respectively). The
rabbit anti-EBOV sGP antibody is able to detect
both the reduced monomer of EBOV sGP as well as
the non-reduced homodimer of EBOV sGP.

ELISA Data:

EBOV sGP and EBOV GPΔTM (cat 0501-001)
proteins were diluted to 400 ng/well in PBS to coat
plates. The rabbit anti-EBOV sGP antibody was
serially diluted, semi-log from 1 µg/mL and
incubated on the coated plates. The washed plates
were detected with anti-rabbit IgG-HRP conjugate
and TMB substrate. The OD650 above shows
strong signals against target EBOV sGP.
Background signals against EBOV GPΔTM were
nearly undetectable