Glucose-1-Phosphate Colorimetric Assay Kit

Glucose-1-Phosphate Colorimetric Assay Kitis available at Gentaur for Next week Delivery.

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Description:

Glucose-1-phosphate (G1P) is an important carbohydrate intermediate in glucose metabolism and storage. In response to hormonal or neural signals, glycogenolysis occurs in liver and muscle tissues where Glucose-1-phosphate is released as the rate-limiting step in glycogen breakdown. Glucose-1-phosphate is subsequently converted to Glucose-6-phosphate by phosphoglucomutase and enters glycolysis. In glycogen synthesis, glucose is transferred to glycogen through the actions of Phosphoglucose isomerase (G6P à G1P), UDPG-pyrophosphorylase (G1P à UDP-glucose) and glycogen synthase (UDP-glucose + glycogen[n] à UDP + glycogen[n+1]). Glucose-1-phosphate is found in virtually all organisms from bacteria to higher plants and animals. Measurement of intracellular G1P levels is crucial for analyzing the carbohydrate metabolic pathways and their kinetic properties. In Glucose-1-phosphate assay, G1P is converted to glucose-6-phosphate by phosphoglucomutase in the presence of Glucose 1,6-biphosphate; glucose-6-phosphate is subsequently oxidized by glucose-6-phosphate dehydrogenase to form NADH which reduces a colorless probe to a colored product with strong absorbance at 450 nm. BioVision’s Glucose-1-phosphate assay kit is rapid, sensitive & easy to use & can detect 1µM to 10 mM G1P. This G1P assay kit can be used for a variety of sample types.

Applications:

Sample Typ:e • Animal tissues: Liver, muscle and heart etc. • Cell culture: Adherent or suspension cells.

Alternate Name: G1P Colorimetric Assay Kit

Features and Benefits:

• Simple procedure; takes ~ 40 minutes • Rapid, convenient and sensitive • Kit contains all necessary reagents for accurate measurement of glucose-1-phosphate levels