Glucose Uptake Colorimetric Assay Kit

Glucose Uptake Colorimetric Assay Kitis available at Gentaur for Next week Delivery.

Non-radioactive, Highly Sensitive Assay.

Description:

Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells. In BioVision’s glucose uptake colorimetric assay kit, the 2-DG6P is oxidized to generate NADPH, which can be determined by an enzymatic recycling amplification reaction. This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well.

Applications:

Sample Typ:e Living cells (suspension and adherent)

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Features and Benefits:

• Simple procedure • Fast and convenient • The assay is easy, non-radioactive, and highly sensitive