Caspase-9 (Active) Antibody

Caspase-9 (Active) Antibodyis available at Gentaur for Next week Delivery.

Description: Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase-9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase-9 (47 kDa) and Apaf-1. The complex processes pro-caspase-9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase-9 further processes other caspases including caspase-3 and caspase-6, to initiate a caspase cascade leading to apoptosis. The affinity purified antibody recognizing the active forms of caspase-9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.

Immunogen Sequence:Synthetic peptide mapping to the N-terminus adjacent to Asp330 of human caspase-9

Target Alternative Name:CASP-9, EC 3.4.22.62, ICE-like apoptotic protease 6,ICE-LAP6,Apoptotic protease Mch-6,Apoptotic protease-activating factor 3, Apaf-3

Species Reactivities:Human

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Formulation:100 µg (0.2 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 0.5% BSA, and 0.02% thimerosal.

Purification: Affinity purified

Application:Western blot, Immunoprecipitation

Positive Control:/

Application And Usages:Western blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). However, the optimal concentrations should be determined individually. The anti-active caspase-9 antibody recognizes only the cleaved caspase-9 (37 kDa). It does not recognize full-length caspase-9 or any other caspases.

Handling:The antibody solution should be gently mixed before use.