Anti-Histone H2AX Rabbit Monoclonal Antibody

Anti-Histone H2AX Rabbit Monoclonal Antibodyis available at Gentaur for Next week Delivery.

Description: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Immunogen Sequence:A peptide corresponding to the C-terminus of human Histone H2AX

Target Alternative Name:H3F3A, H3.3A, H3F3, H3F3B, H3.3B

Species Reactivities:Human

Tag Line:Rabbit monoclonal antibody to detect Histone H2AX

Formulation:In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Purification: Affinity purified using protein A

Application:WB, ICC, ELISA, Multiplex

Positive Control:WB: A375, HEK293, HeLa and SK-MEL-2; ICC: HeLa cells

Application And Usages:Western Blot: 0.5 ug/mL – 2 ug/mL; ICC: 1 ug/mL - 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.2 ug/mL – 1 ug/mL.

Handling:The antibody solution should be gently mixed before use.