Anti-His-Tag Polyclonal Antibody

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SKU:
3998-30T
  • Anti-His-Tag Polyclonal Antibody
  • Anti-His-Tag Polyclonal Antibody
£65.12
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Description

Anti-His-Tag Polyclonal Antibodyis available at Gentaur for Next week Delivery.

Description: A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.

Immunogen Sequence:6xHis synthetic peptide

Target Alternative Name:His tag, HHHHHH tag

Species Reactivities:Detects His-tagged recombinant proteins

Tag Line:/

Formulation:0.5 mg/ml affinity purified rabbit anti-His-Tag polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.5 mM EDTA and 0.03% Proclin

Purification: Affinity purified

Application:Western blot

Positive Control:/

Application And Usages:The antibody can be used for Western blot analysis (1 µg/ml). However, the optimal conditions should be determined by the user.

Handling:The antibody solution should be gently mixed before use.

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Additional Information

Size:
30 µg
Antibody Target:
His-Tag
Category:
His-Tag
Lot Number:
51298
Host:
Rabbit
Antibody Type:
Polyclonal
Isotype:
Rabbit IgG
Concentration:
0.5 mg/ml
Appearance:
Colorless liquid
Form:
Liquid
Storage Condition:
-20 °C
Shipping Condition:
gel pack
Shelf Life:
12 months
Country of Animal Origin:
China
Country of Manufacture:
USA
Usage:
For Research Use Only! Not to be used in humans.
Western Blot Verified:
Verified
Immunocytochemistry Verified:
Not Verified
Immunofluorescence Verified:
Not Verified
Immunoprecipitation Verified:
Not Verified
FACS Verified:
Not Verified
ELISA Verified:
Not Verified
ChIP Verified:
Not Verified
Flow Cytometry Verified:
Not Verified
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