Anti-His-Tag Polyclonal Antibody

Anti-His-Tag Polyclonal Antibodyis available at Gentaur for Next week Delivery.

Description: A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.

Immunogen Sequence:6xHis synthetic peptide

Target Alternative Name:His tag, HHHHHH tag

Species Reactivities:Detects His-tagged recombinant proteins

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Formulation:0.5 mg/ml affinity purified rabbit anti-His-Tag polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.5 mM EDTA and 0.03% Proclin

Purification: Affinity purified

Application:Western blot

Positive Control:/

Application And Usages:The antibody can be used for Western blot analysis (1 µg/ml). However, the optimal conditions should be determined by the user.

Handling:The antibody solution should be gently mixed before use.