Anti-5-Methylcytosine Antibody

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SKU:
A1294-50
€415
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Description

Anti-5-Methylcytosine Antibodyis available at Gentaur for Next week Delivery.

Description: 5-methylcytosine (5-mC) is a modified base present in nucleic acids of plants and vertebrates. DNA methylation is a post-replicative process involved in the establishment of genomic imprinting, in the control of gene expression and of differentiation. Carcinogenesis is associated with alterations of the DNA methylation pattern: a global hypomethylation is often detected in tumor tissues when compared to their normal counterpart. Simultaneously local hypermethylation sites are observed.

Immunogen Sequence:Modified ribonucleoside, specific for the presence of a methyl group on carbon 5 of the pyrimidine ring

Target Alternative Name:5-mc, 5mc

Species Reactivities:Human, Mouse, Rat

Tag Line:Monoclonal antibody to detect 5-Methylcytosine

Formulation:Phosphate Buffer 10mM - NaCl 0.15 M - pH 7.4

Purification: Affinity purified

Application:WB, ELISA, MeDIP/ChIP, IF, , IHC, FC

Positive Control:/

Application And Usages:WB; 1:250, ELISA: 1:10000, MeDIP/ChIP: 1-2 µg per IP, IF: 1:500, IHC Perform heat mediated antigen retrieval before commencing with IHC staining protocol, ICC; 0.5-5 µg/ml, FC; 10µl of working dilution to label 10 x 106 cells in 100µl

Handling:The antibody solution should be gently mixed before use

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Additional Information

Size:
50 µg
Antibody Target:
5-mc
Category:
Epigenetics
Lot Number:
N/A
Host:
Mouse
Antibody Type:
Monoclonal
Isotype:
Mouse IgG1, Lambda
Appearance:
Colorless liquid
Form:
Liquid
Storage Condition:
-20 °C
Shipping Condition:
gel pack
Shelf Life:
12 months
Country of Animal Origin:
USA
Country of Manufacture:
USA
Usage:
For Research Use Only! Not to be used in humans
Western Blot Verified:
Not Verified
Immunocytochemistry Verified:
Not Verified
Immunofluorescence Verified:
Not Verified
Immunoprecipitation Verified:
Not Verified
FACS Verified:
Not Verified
ELISA Verified:
Not Verified
ChIP Verified:
Not Verified
Dot Blot Verified:
Not Verified
Flow Cytometry Verified:
Not Verified
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