Anti-5-Methylcytosine Antibody

Anti-5-Methylcytosine Antibodyis available at Gentaur for Next week Delivery.

Description: 5-methylcytosine (5-mC) is a modified base present in nucleic acids of plants and vertebrates. DNA methylation is a post-replicative process involved in the establishment of genomic imprinting, in the control of gene expression and of differentiation. Carcinogenesis is associated with alterations of the DNA methylation pattern: a global hypomethylation is often detected in tumor tissues when compared to their normal counterpart. Simultaneously local hypermethylation sites are observed.

Immunogen Sequence:Modified ribonucleoside, specific for the presence of a methyl group on carbon 5 of the pyrimidine ring

Target Alternative Name:5-mc, 5mc

Species Reactivities:Human, Mouse, Rat

Tag Line:Monoclonal antibody to detect 5-Methylcytosine

Formulation:Phosphate Buffer 10mM - NaCl 0.15 M - pH 7.4

Purification: Affinity purified

Application:WB, ELISA, MeDIP/ChIP, IF, , IHC, FC

Positive Control:/

Application And Usages:WB; 1:250, ELISA: 1:10000, MeDIP/ChIP: 1-2 µg per IP, IF: 1:500, IHC Perform heat mediated antigen retrieval before commencing with IHC staining protocol, ICC; 0.5-5 µg/ml, FC; 10µl of working dilution to label 10 x 106 cells in 100µl

Handling:The antibody solution should be gently mixed before use