Streptomycin ELISA Kit

Streptomycin ELISA Kit


Streptomycin is an antibiotic (antimycobacterial) drug, the first of a class of drugs called aminoglycosides to be discovered, and it was the
first effective treatment for tuberculosis. It is derived from the actinobacterium Streptomyces griseus.

Streptomycin is a bactericidal antibiotic. Adverse effects of this medicine are ototoxicity, nephrotoxicity, fetal auditory toxicity, and neuromuscular paralysis.

BioVision’s Streptomycin ELISA kit is a competitive ELISA assay for the quantitative measurement of Streptomycin in tissue, honey and milk.

The density of color is proportional to the amount of Streptomycin captured from the samples.

Assay Protocol:

  1. Prepare all reagents, samples and standards as instructed in section VII.
  2. Add 50 μl standards or samples into marked well. Add 50 μl antibody working solution into each well.
  3. Oscillate the plate for 5 sec, cover the well and incubate for 30 min at RT (25°C).
  4. Discard solution, wash plate 5 times with 1X Wash Solution. Wash by filling each well with Wash Buffer (250 μl) using a multi-channel
  5. pipette or autowasher. Let it soak for 1 min, and then remove all residual wash-liquid from the wells. After the last wash, remove any
  6. remaining Wash Buffer by aspirating or decanting. Clap the plate on absorbent filter papers or other absorbent materials.
  7. Add 100 µl Enzyme conjugate into each well; avoid the light to incubate for 30 min at 25°C.
  8. Pipette 50 μl Substrate A solution, then pipette 50 μl Substrate B solution to each well, oscillate gently for 5 sec, avoid the light
  9. preservation for 15 min at RT.
  10. Add 50 μl Stop Solution to each well and