Description
WISP-3, Human Recombinant is available at Gentaur for Next week delivery.
Belongs to CCN family of cysteine rich regulatory proteins.
Biomolecule/Target: WISP-3
Alternates names: WNT-1 inducible signaling pathway protein-3, CCN6, WISP-3
Synonyms: WNT-1 inducible signaling pathway protein-3, CCN6, WISP-3
Background Information: WISP-3 is a member of the CCN family of secreted cysteine rich regulatory proteins. It is predominantly expressed in the adult kidney, testis and fetal kidney and weaker expression in placenta, ovary, prostate, small intestine, and skeletally-derived cells. WISP-3 is required for normal postnatal skeletal growth and cartilage homeostasis. Human WISP-3 is a 36.8 kDa protein containing 331 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor-C (VWFC) domain, the thrombospondin type-1 repeat (TSP type-1) domain, and a C-terminal cysteine knot-like (CTCK) domain.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in 10 mM Acetic Acid to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
NCBI Gene Symbol: WISP3
NCBI Accession: O95389
Additional Information
Size: |
5 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E.coli |
Purity by SDS-PAGE: |
≥ 98% |
Assay: |
SDS-PAGE |
Purity: |
≥ 98% |
Assay 2: |
HPLC |
Endotoxin Level: |
<0.1 EU/μg |
Biological activity: |
The ED50 was determined by the dose-dependent proliferation of the MCF-7 cell line. The expected ED50 for this effect is 0.2-0.3 µg/ml. |
Results: |
N/A |
Molecular Weight: |
36.8 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |