Description
Urokinase, human recombinant is available at Gentaur for Next week delivery.
A potent marker of invasion and metastasis in a variety of human cancers
Biomolecule/Target: Urokinase
Alternates names: Two chain urokinase-type plasminogen activator (tcuPA), PLAU, ATF, UPA, URK, u-PA, BDPLT5，QPD
Synonyms: Two chain urokinase-type plasminogen activator (tcuPA), PLAU, ATF, UPA, URK, u-PA, BDPLT5，QPD
Background Information: Urokinase or Urokinase-type plasminogen activator (uPA) is a serine protease (EC 3.4.21.73). It is secreted as a single-chain zymogen, pro-Urokinase, possessing little or no intrinsic enzymatic activity. The single chain zymogen is converted into the active two chain enzyme (tcuPA) by cleavage of the bond between Lys158 and Ile159. After activation, Urokinase specifically cleaves the proenzyme plasminogen to form the active enzyme plasmin. The active plasmin then catalyzes the breakdown of fibrin polymers of blood clots. Urokinase is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing. Additionally, it is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.
Reconstitution Instructions: Briefly spin down the vial and reconstitute in water to 0.5-1 mg/ml and store at –80°C.
NCBI Gene Symbol: PLAU
Gene ID: 5328
NCBI Accession: P00749
Additional Information
Size: |
500 µg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. coli. |
Purity by SDS-PAGE: |
≥90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
The enzyme is fully active as seen from its ability to cleave a fluorogenic substrate N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC) (Cat # K728-100). |
Biological activity: |
>1500 mU/mg (1 U = Digestion of 1 µmole of Z-GGR-AMC substrate in 1 min at 37ºC.) |
Results: |
>1500 mU/mg (1 U = Digestion of 1 µmole of Z-GGR-AMC substrate in 1 min at 37ºC.) |
Molecular Weight: |
The active Urokinase is a two-chain glycoprotein and has a calculated MW of 49.3 kDa. DTT-reduced Protein migrates as two bands corresponding to the two chains with the molecular mass of 28.3 and 21 kDa. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized powder |
Handling: |
Centrifuge the vial prior to opening. |