Description
TSG, human recombinant is available at Gentaur for Next week delivery.
Inhibits BMP activity by binding directly to BMP proteins
Biomolecule/Target: TSG
Alternates names: Twisted Gastrulation Protein
Synonyms: Twisted Gastrulation Protein
Background Information: Twisted Gastrulation Protein (TSG) is a secreted BMP binding protein structurally related to the BMP antagonists Chordin and Noggin. TSG can inhibit BMP activity by binding directly to BMP proteins, and can act either as a BMP4 agonist or antagonist (depending on the specific biochemical environment) by binding to the BMP4/Chordin complex. Recombinant human TSG is a 198 amino acid 22.2 kDa protein containing the BMP/TGFβ binding portion of the full length TSG protein.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
NCBI Gene Symbol: TSG
Gene ID: 57045
NCBI Accession: Q9GZX9
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. Coli |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
< 0.1 ng/μg of protein (<1EU/μg). |
Activity (Specifications/test method): |
Determined by its ability to neutralize BMP-6 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is ≤ 2.0 μg/ml of TSG. |
Biological activity: |
Determined by its ability to neutralize BMP-6 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is ≤ 2.0 μg/ml of TSG. |
Results: |
N/A |
Molecular Weight: |
22.2 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized powder |
Handling: |
Centrifuge the vial prior to opening. |