SGK1, Active

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SKU:
7748-5
€185
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Description

SGK1, Active is available at Gentaur for Next week delivery.

Regulates the myo-inositol transporter during osmotic stress

Biomolecule/Target:

Alternates names: SGK, serum/glucocorticoid regulated kinase 1

Synonyms: SGK, serum/glucocorticoid regulated kinase 1

Background Information: SGK1 is a member of the serum- and glucocorticoid-induced protein kinase that is activated in vitro by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and in vivo in response to signals that activate phosphatidylinositol (PI) 3-kinase (1). SGK1, mRNA is expressed in all tissues and the levels of SGK1 mRNA is increased by stimulation with serum or dexamethasone. SGK1 promotes cell survival by phosphorylating and inactivating FKHRL1. SGK and Akt display differences with respect to the efficacy with which they phosphorylate the three regulatory sites on FKHRL1. While both kinases can phosphorylate Thr-32, SGK1 displays a marked preference for Ser-315 whereas Akt favors Ser-253. These findings suggest that SGK1 and Akt may coordinately regulate the function of FKHRL1 by phosphorylating this transcription factor at distinct sites. Like PKB, SGK1 preferentially phosphorylate Ser and Thr residues that lie in Arg-Xaa-Arg-Xaa-Xaa-Ser/Thr motifs. SGK1 gene has recently been identified as an important aldosterone-induced protein kinase that mediates trafficking of the renal epithelial Na(+) channel (ENaC) to the cell membrane. Thus, SGK1 is an appealing candidate for blood pressure regulation and possibly essential hypertension (3).

Reconstitution Instructions: N/A

NCBI Gene Symbol: SGK1

Gene ID: 6446

NCBI Accession: O00141

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Additional Information

Size:
5 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Recombinant:
Yes
Source:
Baculovirus (Sf9 insect cells)
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
N/A
Activity (Specifications/test method):
65 nmol phosphate incorporated into Akt/SGK substrate per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Biological activity:
N/A
Results:
N/A
Molecular Weight:
73.0 kDa
Storage Temperature:
-80°C
Shelf Life:
12 months
Concentration:
5µg/50 µl
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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