sCD100/Semaphorin-4D, Human Recombinant

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SKU:
7126-50
£135.37
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Description

sCD100/Semaphorin-4D, Human Recombinant is available at Gentaur for Next week delivery.

Play a role in maintaining homeostasis in the immune system

Biomolecule/Target: sCD100

Alternates names: Semaphorin-4D, BB18, A8, GR3

Synonyms: Semaphorin-4D, BB18, A8, GR3

Background Information: The Semaphorins are a large family of phylogenetically conserved proteins that play a pivotal role in maintaining homeostasis in the immune system. Twenty members of this family have been identified and categorized into eight subclasses based on sequence similarity and distinctive structural features. CD100, also known as Sema4D, is a 150 kDa transmembrane class IV semaphorin. Studies have shown that CD100 can induce monocyte migration, T-cell activation, and B-cell survival, as well as T/B cell and T/DC “cooperation”. The CD100 precursor contains 862 amino acids, including a 21 aa. signal sequence, a 713 aa. extracellular domain, a 21 aa transmembrane sequence, and a 107 aa cytoplasmic region. The extracellular sequence contains several structural features, including a 479 aa “sema” domain, a 79 aa. Ig-like sequence, and a 52 aa “Plexin-type repeat”. Recombinant soluble CD100 is a 78.9 kDa protein comprising the extracellular domain of CD100 (711 amino acids). SDS-PAGE analysis run under non-reducing conditions shows a mixture of disulfide linked dimer and monomer.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

NCBI Gene Symbol: CD100

Gene ID: 10507

NCBI Accession: Q92854

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Additional Information

Size:
50 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
CHO cells
Purity by SDS-PAGE:
≥97%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
< 0.1 ng/μg of protein (<1EU/μg).
Activity (Specifications/test method):
Measured by its ability to inhibit chemokine (hMCP-3) induced human monocyte migration.
Biological activity:
Measured by its ability to inhibit chemokine (hMCP-3) induced human monocyte migration.
Results:
N/A
Molecular Weight:
78.9 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized powder
Handling:
Centrifuge the vial prior to opening.
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