Description
Red Fluorescent Protein - R-PE (R-Phycoerythrin) is available at Gentaur for Next week delivery.
Fluorescent protein which can be cross linked or conjugated to other molecules to make fluorescent probes.
Biomolecule/Target: R-PE
Alternates names: RFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin
Synonyms: RFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin
Background Information: R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. The broad excitation spectrum provides the advantage for multi-color immunofluorescent staining or cell sorting. R-PE consists of a, b and g subunits and is present as (ab) 6g. R-PE and the closely related BPE are the most intensely fluorescent phycobiliproteins having orange fluorescence. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates.
Reconstitution Instructions: Before use, centrifuge the R-PE suspension at 10,000g for 10 min at 4C. Discard the supernatant and resuspend the pellet (R-PE) into the desired buffer. R-PE is stored in a salt solution and it must be extensively dialyzed (Can use BioVision’s Dialyzer tubes) or desalted in columns (BioVision Cat # 6564-25) prior to use, to remove any remaining ammonium sulphate.
NCBI Gene Symbol: R-PE
Gene ID: N/A
NCBI Accession: N/A
Additional Information
Size: |
5 mg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Red algae |
Recombinant: |
No |
Source: |
Febico’s proprietary red algae |
Purity by SDS-PAGE: |
A566/A280 ≥ 5.5 |
Assay: |
Absorbance |
Purity: |
A566/A498 ≤1.5 |
Assay 2: |
Absorbance |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
N/A |
Biological activity: |
N/A |
Results: |
N/A |
Molecular Weight: |
240 kDa |
Storage Temperature: |
2-8°C |
Shelf Life: |
60 months |
Concentration: |
N/A |
Appearance: |
Suspension |
Handling: |
Centrifuge the vial prior to opening. |