Description
Recombinant E. coli, Glucokinase is available at Gentaur for Next week delivery.
An important initiation enzyme in glucose catabolism (glycolysis) that catalyzes the phosphorylation of glucose to yield glucose 6-phosphate (G6P).
Biomolecule/Target: glk
Alternates names: ECK2384, JW2385, glk
Synonyms: ECK2384, JW2385, glk
Background Information: glk belongs to the bacterial glucokinase family. This protein is not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. Recombinant E.coli glk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: glk
Gene ID: 946858
NCBI Accession: 946858
Additional Information
Size:  | 
                                                    50 μg  | 
                                                
Country of Manufacturing Origin:  | 
                                                    USA  | 
                                                
Country of Animal Origin:  | 
                                                    USA  | 
                                                
Gene Source:  | 
                                                    E. coli  | 
                                                
Recombinant:  | 
                                                    Yes  | 
                                                
Source:  | 
                                                    E. coli  | 
                                                
Purity by SDS-PAGE:  | 
                                                    > 95%  | 
                                                
Assay:  | 
                                                    SDS-PAGE  | 
                                                
Purity:  | 
                                                    N/A  | 
                                                
Assay 2:  | 
                                                    N/A  | 
                                                
Endotoxin Level:  | 
                                                    N/A  | 
                                                
Activity (Specifications/test method):  | 
                                                    Measured by increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP.  | 
                                                
Biological activity:  | 
                                                    Specific activity is >70 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP.  | 
                                                
Results:  | 
                                                    >70 units/mg  | 
                                                
Molecular Weight:  | 
                                                    37.1 kDa  | 
                                                
Storage Temperature:  | 
                                                    -20°C  | 
                                                
Shelf Life:  | 
                                                    12 months  | 
                                                
Concentration:  | 
                                                    N/A  | 
                                                
Appearance:  | 
                                                    Liquid  | 
                                                
Handling:  | 
                                                    Centrifuge the vial prior to opening.  |