Description
Recombinant E. coli, Glucokinase is available at Gentaur for Next week delivery.
An important initiation enzyme in glucose catabolism (glycolysis) that catalyzes the phosphorylation of glucose to yield glucose 6-phosphate (G6P).
Biomolecule/Target: glk
Alternates names: ECK2384, JW2385, glk
Synonyms: ECK2384, JW2385, glk
Background Information: glk belongs to the bacterial glucokinase family. This protein is not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. Recombinant E.coli glk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: glk
Gene ID: 946858
NCBI Accession: 946858
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
E. coli |
Recombinant: |
Yes |
Source: |
E. coli |
Purity by SDS-PAGE: |
> 95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Measured by increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. |
Biological activity: |
Specific activity is >70 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. |
Results: |
>70 units/mg |
Molecular Weight: |
37.1 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |