Recombinant E. coli, D-lactate Dehydrogenase

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SKU:
P1053-50
€185
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Description

Recombinant E. coli, D-lactate Dehydrogenase is available at Gentaur for Next week delivery.

Biomolecule/Target: ldhA

Alternates names: ECK1377, hslF, hslI, htpH, JW1375

Synonyms: ECK1377, hslF, hslI, htpH, JW1375, IdhA

Background Information: Idha, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are (D)-lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c. Recombinant E.coli IdhA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Reconstitution Instructions: N/A

NCBI Gene Symbol: ldhA

Gene ID: 946315

NCBI Accession: P52643

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Additional Information

Size:
50 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
E. coli
Recombinant:
Yes
Source:
E. coli
Purity by SDS-PAGE:
> 95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
N/A
Activity (Specifications/test method):
Measured by its ability to convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
Biological activity:
Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
Results:
> 200 units/mg
Molecular Weight:
39.1 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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