R-Spondin-2, human recombinant

(No reviews yet) Write a Review
SKU:
7190-50
£135.37
Frequently bought together:

Description

R-Spondin-2, human recombinant is available at Gentaur for Next week delivery.

Regulates the proliferation and differentiation of certain stem cell populations

Biomolecule/Target: R-Spondin-2

Alternates names: Roof plate-specific Spondin 2, Rspo2

Synonyms: Roof plate-specific Spondin 2, Rspo2

Background Information: The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in a manner that causes the stabilization and resulting accumulation of the intracellular signaling protein, β-catenin. This activity effectively activates and increases the subsequent nuclear signaling of β-catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/β-catenin signaling can act as an inducer of the transformed phenotype, and can also regulate the proliferation and differentiation of certain stem cell populations. Recombinant human R-Spondin-2 is a 24.4 kDa protein consisting of 212 amino acid residues. Due to glycosylation, R-Spondin-2 migrates at an apparent molecular weight of approximately 30.0 kDa by SDS PAGE analysis under reducing conditions.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

NCBI Gene Symbol: RSPO2

Gene ID: 340419

NCBI Accession: Q6UXX9

View AllClose

Additional Information

Size:
50 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
CHO Cells
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
< 0.1 ng/μg of protein (<1EU/μg).
Activity (Specifications/test method):
R-Spondin-2 enhances BMP-2 mediated differentiation of MC3T3-E1 cells.
Biological activity:
R-Spondin-2 enhances BMP-2 mediated differentiation of MC3T3-E1 cells.
Results:
N/A
Molecular Weight:
30.0 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized powder
Handling:
Centrifuge the vial prior to opening.
View AllClose

0 Reviews

View AllClose